International Streptomyces Project-2 Medium (ISP-2)
ISP-2 is a rich medium that is routinely used for cultivating Streptomyces lydicus along with various other plant root associated strains. It contains malt extract and yeast extract, with dextrose as a carbon source; exact composition will vary between batches.
Many Streptomyces spp. grow well on ISP-2, but developmental and/or antibiotic production phenotypes may differ compared to growth on other media (e.g. SFM).
- 4 g Yeast extract powder
- 10 g Malt extract powder
- 4 g Dextrose
- 20 g Agar
- 1 L dH2O
- For solid medium, add 2g of Agar to each flask
- Add the rest of the components to a beaker with the dH2O
- Add 100ml to each flask
- Streptomyces maintenance
- Spore titres
- Re-isolation experiments
- Selection for resistance to salt-sensitive antibiotics
For growth in liquid culture, ISP-2 can be made without the agar.