From ActinoBase

Minimal liquid medium (NMMP)

NMMP medium is used to obtain dispersed growth of Streptomyces sp. with specific carbon sources.[2]


Make up the following solutions:

Minor elements solution: (per litre)

  • 1g ZnSO4*7H2O
  • 1g FeSO4*7H2O
  • 1g MnCl2*4H2O
  • 1g CaCl2, anhydrous

Phosphate buffer

  • Mix 0.1M solutions of NaH2PO4 and K2HPO4 to give pH 6.8

Carbon source

  • 20% solution, sterilized separately


  • 2 g (NH4)2SO4
  • 5 g Difco Casaminoacids
  • 0.6g MgSO4*7H2O
  • 50g PEG 6000
  • 1 mL minor elements solution
  • 800 mL ddH2O


  1. Make up the NMMP solution described above
  2. Dispense in 80 mL aliquots
  3. Autoclave
  4. Make up the phosphate buffer solution, carbon source, and any other required growth factors, and autoclave these separately

Just before using, add:

  • 15 mL NaH2PO4/K2HPO4 buffer (0.1M, pH 6.8)
  • 2.5 mL carbon source (20%)
  • 2.5 mL any other required growth factors (add 2.5 mL sterile water if none)


  • Routine growth of Streptomyces sp. in liquid culture


  • Using PEG-6000 and adding stainless steel springs in the culture flasks allows for good dispersed growth of many Streptomyces strains.[1]
  • Agar can be added (20 g/L) to make solid NMMP.[3]
  • A modified version of NMMP suitable for 14N/15N labelling has been developed.[4]


[1] Hodgson (1982). Glucose Repression of Carbon Source Uptake and Metabolism in Streptomyces coelicolor A3(2) and its Perturbation in Mutants Resistant to 2-Deoxyglucose. Journal of General Microbiology 128: 2417-2430.

[2] Kieser, T., Bibb, M.J., Buttner, M.J., Chater, K.F. and Hopwood, D.A. (2000) Practical Streptomyces Genetics: John Innes Foundation, Norwich Research Park, Colney, Norwich NR4 7UH, UK

[3] Rico, S., Yepes, A., Rodríguez, H., Santamaría, J., Antoraz, S., Krause, E. M., … Santamaría, R. I. (2014). Regulation of the AbrA1/A2 two-component system in Streptomyces coelicolor and the potential of its deletion strain as a heterologous host for antibiotic production. PloS one, 9(10), e109844. doi:10.1371/journal.pone.0109844

[4] Świątek, M. A., Gubbens, J., Bucca, G., Song, E., Yang, Y. H., Laing, E., … van Wezel, G. P. (2013). The ROK family regulator Rok7B7 pleiotropically affects xylose utilization, carbon catabolite repression, and antibiotic production in Streptomyces coelicolor. Journal of bacteriology, 195(6), 1236–1248. doi:10.1128/JB.02191-12