OT

OT(oligotrophic agar)

Oligotrophic agar is an extremely nutritionally-poor medium useful for isolating slow-growing actinomyces e.g. from soil.

Preparation

Per litre:

• 0.05g Yeast Extract
• 0.5g tryptone
• 0.1g sodium glycerophosphate
• 12g agar

Instructions

1. Mix well all the components in 1000 mL of distilled water.
2. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
3. Mix well and pour into sterile Petri dishes.

Uses

• Isolation experiments