Uploads by MFeeney

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File list
Date Name Thumbnail Size Description Versions
11:04, 6 May 2020 SsDNA recombineering oligos.png (file) 8 KB oligonucleotide design for ssDNA recombineering 1
10:16, 6 May 2020 SsDNA recombineering protocol.png (file) 70 KB figure illustrating two key steps in the ssDNA recombineering protocol 1
10:13, 6 May 2020 SsDNA diagnostic pcr.png (file) 60 KB description of restriction polymorphism and mismatch PCR approaches to identify the mutant allele generated by ssDNA recombineering 1
09:47, 5 May 2020 PhiC31 serial dilution.jpg (file) 122 KB Plaque assay: serial dilution of phiC31 on pglW- S. coelicolor 1
08:34, 5 May 2020 PhiC31 plaque assay.jpg (file) 509 KB PhiC31 plaque assay on a pglW- strain of S. coelicolor 1