1/2 MS+B5: Difference between revisions
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(Created page with "==Preparation== Per Litre: *2.205 MS Salts and Gamborg B5 Vitamins (Duchefa M0231) *1 L ddH2O *5M KOH *Bacto Agar '''Instructions''' #Dissolve 2.2g MS Salts + Gamborg B5 Vit...") |
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For solid media: | For solid media: | ||
Add 12 g Bacto Agar | |||
==Uses== | ==Uses== | ||
*[[Growing Marchantia from gemma spores]] | *[[Growing Marchantia from gemma spores]] |
Latest revision as of 14:18, 2 November 2021
Preparation
Per Litre:
- 2.205 MS Salts and Gamborg B5 Vitamins (Duchefa M0231)
- 1 L ddH2O
- 5M KOH
- Bacto Agar
Instructions
- Dissolve 2.2g MS Salts + Gamborg B5 Vitamin mix in 950ml ddH2O
- Adjust pH to 6.7 with 5M KOH
- Make up to final volume with water (for 1L, add the remaining ≈50ml ddH2O)
- Divide into batches (in bottles containing Bacto Agar if required)
- Autoclave until golden brown
For solid media: Add 12 g Bacto Agar