1/2 MS+B5

Preparation

Per Litre:

• 2.205 MS Salts and Gamborg B5 Vitamins (Duchefa M0231)
• 1 L ddH2O
• 5M KOH
• Bacto Agar

Instructions

1. Dissolve 2.2g MS Salts + Gamborg B5 Vitamin mix in 950ml ddH2O
2. Adjust pH to 6.7 with 5M KOH
3. Make up to final volume with water (for 1L, add the remaining ≈50ml ddH2O)
4. Divide into batches (in bottles containing Bacto Agar if required)
5. Autoclave until golden brown

For solid media:

1. Add 12 g Bacto Agar

Uses

• Growing Marchantia from gemma spores