Antibiotic stocks and working concentrations: Difference between revisions
From ActinoBase
(Created page with "{| class="wikitable" ! style="font-weight:bold; background-color:#c0c0c0;" | Antibiotic ! style="font-weight:bold; background-color:#c0c0c0;" | Stock [mg/ml] ! style="font-wei...") |
No edit summary |
||
| Line 1: | Line 1: | ||
The following antibiotics can be used for selection at these concentrations in most <em>Streptomyces</em> sp. (If working with a new species, you should always first check whether it has intrinsic resistance to an antibiotic before attempting to use it.) | |||
{| class="wikitable" | {| class="wikitable" | ||
! style="font-weight:bold; background-color:#c0c0c0;" | Antibiotic | ! style="font-weight:bold; background-color:#c0c0c0;" | Antibiotic | ||
| Line 29: | Line 31: | ||
|- | |- | ||
| Nalidixic acid | | Nalidixic acid | ||
| style="text-align: center;" | 25 in | | style="text-align: center;" | 25 in 0.2N NaOH | ||
| style="text-align: center;" | 25 | | style="text-align: center;" | 25 | ||
|} | |} | ||
<strong>Notes:</strong> | |||
*Hygromycin selection is salt-sensitive; on low-salt media such as [[DNA]], 50 µg /ml (final concentration) is sufficient for selection. | |||
*Carbenicillin is more stable than ampicillin, but care should still be taken to avoid excessive freeze/thaw cycles which could result in degradation of the antibiotic. | |||
Revision as of 13:50, 31 July 2019
The following antibiotics can be used for selection at these concentrations in most Streptomyces sp. (If working with a new species, you should always first check whether it has intrinsic resistance to an antibiotic before attempting to use it.)
| Antibiotic | Stock [mg/ml] | Final concentration [µg /ml] |
|---|---|---|
| Apramycin | 100 in dH2O | 50 |
| Hygromycin | 50 in dH2O | 100 |
| Kanamycin | 50 in dH2O | 50 |
| Chloramphenicol | 25 in 100% EtOH | 25 |
| Carbenicillin | 100 in dH2O | 100 |
| Thiostrepton | 50 in 100% DMSO | 50 |
| Nalidixic acid | 25 in 0.2N NaOH | 25 |
Notes:
- Hygromycin selection is salt-sensitive; on low-salt media such as DNA, 50 µg /ml (final concentration) is sufficient for selection.
- Carbenicillin is more stable than ampicillin, but care should still be taken to avoid excessive freeze/thaw cycles which could result in degradation of the antibiotic.
