CM

CM (Complete Medium)

Preparation

Per litre:

• 5g K₂HPO₄
• 0.5g NaCl
• 0.5g MgSO₄*7H₂O
• 2g Difco Bacto-peptone
• 1.5g Difco Casaminoacids
• 50mg eachL-histidine, L-proline, L-tryptophan, and L-tyrosine^*
• 5mL Yeast nucleic acid hydrolysate
• 1mL vitamin solution
• 25g glucose
• 10g agar
• 1L dH₂O

Yeast nucleic acid hydrolysate

Vitamin solution

• 100mg riboglavine
• 100mg nicotinamide
• 10mg p-aminobenzoic acid
• 50mg pyridoxine HCl
• 50mg thiamine HCl
• 20 mg biotin
• 100mL water

Instructions

1. Make up the yeast nucleic acid hydrolysate according to the directions above: do not sterilize. Make up the vitamin solution following the recipe above and sterilize by autoclaving (115˚C, 10 min).

Dissolve all of the ingredients, except the agar and the vitamin solution, but including the glucose, in the distilled water

1. Adjust the pH to 7.2 with 1N HCl
2. Add the vitamin solution
3. Dispense 200ml into flasks each containing 2g agar
4. Autoclave

References

Hopwood DA. Genetic analysis and genome structure in Streptomyces coelicolor. Bacteriol Rev. 1967;31(4):373–403.

Kieser, T., Bibb, M.J., Buttner, M.J., Chater, K.F. and Hopwood, D.A. (2000) Practical Streptomyces Genetics: John Innes Foundation, Norwich Research Park, Colney, Norwich NR4 7UH, UK