Difference between revisions of "CRISPR/Cas9-mediated genome editing"
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Can be used for precise CRISPR/Cas9-mediated genome engineering of <em>Streptomyces</em> strains. Mutations made have ranged from 1-100kbp deletions, precise codon changes to alter amino acids and insertions to add Flag-tags to proteins encoded at their native loci. Several different plasmids have been constructed and published and are listed below. Click the links to find out more. | Can be used for precise CRISPR/Cas9-mediated genome engineering of <em>Streptomyces</em> strains. Mutations made have ranged from 1-100kbp deletions, precise codon changes to alter amino acids and insertions to add Flag-tags to proteins encoded at their native loci. Several different plasmids have been constructed and published and are listed below. Click the links to find out more. | ||
| − | [[pCRISPomyces2]] | + | [[pCRISPomyces2]] [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4459934/ Cobb et al 2014 4:723–728] |
Revision as of 10:05, 5 August 2019
Can be used for precise CRISPR/Cas9-mediated genome engineering of Streptomyces strains. Mutations made have ranged from 1-100kbp deletions, precise codon changes to alter amino acids and insertions to add Flag-tags to proteins encoded at their native loci. Several different plasmids have been constructed and published and are listed below. Click the links to find out more.
