Cloning, Transformation and Expression of Gene Clusters: Difference between revisions

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[[Conjugation using ET12567/pUZ8002]]
[[Conjugation using ET12567/pUZ8002]]


[https://academic.oup.com/nar/article/24/3/536/2359981 High Efficiency 5 Minute Transformation of Escherichia coli]
[[Heterologous expression of gene clusters]]


[[Heterologous expression of gene clusters]]
[[PAC cloning]]
 
[[Protoplasts Formation, Regeneration and Transformation]]


[[TAR cloning]]
[[TAR cloning]]


[[Tri-parental mating]] to generate ET12567 strains for the conjugation of large PAC clones or kanamycin marked vectors into ''Streptomyces''.
[[Tri-parental mating]] to generate ET12567 strains for the conjugation of large PAC clones or kanamycin marked vectors into ''Streptomyces''.

Latest revision as of 12:40, 4 September 2019

Conjugation using ET12567/pUZ8002

Heterologous expression of gene clusters

PAC cloning

Protoplasts Formation, Regeneration and Transformation

TAR cloning

Tri-parental mating to generate ET12567 strains for the conjugation of large PAC clones or kanamycin marked vectors into Streptomyces.