Difference between revisions of "Cloning, Transformation and Expression of Gene Clusters"
From ActinoBase
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[[Conjugation using ET12567/pUZ8002]] | [[Conjugation using ET12567/pUZ8002]] | ||
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| + | [[Heterologous expression of gene clusters]] | ||
[https://academic.oup.com/nar/article/24/3/536/2359981 High Efficiency 5 Minute Transformation of Escherichia coli] | [https://academic.oup.com/nar/article/24/3/536/2359981 High Efficiency 5 Minute Transformation of Escherichia coli] | ||
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[[TAR cloning]] | [[TAR cloning]] | ||
[[Tri-parental mating]] to generate ET12567 strains for the conjugation of large PAC clones or kanamycin marked vectors into ''Streptomyces''. | [[Tri-parental mating]] to generate ET12567 strains for the conjugation of large PAC clones or kanamycin marked vectors into ''Streptomyces''. | ||
Revision as of 14:29, 7 August 2019
Conjugation using ET12567/pUZ8002
Heterologous expression of gene clusters
High Efficiency 5 Minute Transformation of Escherichia coli
Tri-parental mating to generate ET12567 strains for the conjugation of large PAC clones or kanamycin marked vectors into Streptomyces.
