DNA: Difference between revisions
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*4g Difco Nutrient Broth Powder | *4g Difco Nutrient Broth Powder | ||
*10g Agar | *10g Agar | ||
*1L | *1L dH<sub>2</sub>O | ||
'''Instructions''' | '''Instructions''' | ||
#For solid medium, add 0.8g of DNB powder into each 250ml conical flask | #For solid medium, add 0.8g of DNB powder into each 250ml conical flask | ||
#Add 2g of Agar | #Add 2g of Agar | ||
#Add 200ml | #Add 200ml dH<sub>2</sub>O | ||
#Autoclave | #Autoclave | ||
Revision as of 12:57, 2 July 2019
Difco Nutrient Agar (DNA)
Difco Nutrient Agar is a rich medium commercially available from BD and routinely used for cultivating many non-fastidious organisms. It contains beef extract and peptones; exact composition will vary between batches.
Many Streptomyces sp. grow well on DNA, but developmental and/or antibiotic production phenotypes may differ compared to growth on other media (e.g. SFM).
Preparation
Per Litre:
- 4g Difco Nutrient Broth Powder
- 10g Agar
- 1L dH2O
Instructions
- For solid medium, add 0.8g of DNB powder into each 250ml conical flask
- Add 2g of Agar
- Add 200ml dH2O
- Autoclave
Uses
- Streptomyces Colony PCR
- Spore titres
- Re-isolation experiments
- Selection for resistance to salt-sensitive antibiotics
Notes
For growth in liquid culture, DNB can be made without the agar.
