IMA: Difference between revisions
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==Notes== | ==Notes== | ||
*Because the agar and instant mashed potato are not very soluble, IMA should be made up in small 100 mL batches (do not attempt to make up 1 L and aliquot into flasks for autoclaving) | *Because the agar and instant mashed potato are not very soluble, IMA should be made up in small 100 mL batches (do not attempt to make up 1 L and aliquot into flasks for autoclaving) | ||
*[[''Streptomyces scabies''] grows particularly well on IMA. | *[[''Streptomyces scabies'']] grows particularly well on IMA. | ||
*Strains of [[''Streptomyces coelicolor''] that fail to sporulate very well on [[SFM]] may sporulate better on IMA. | *Strains of [[''Streptomyces coelicolor'']] that fail to sporulate very well on [[SFM]] may sporulate better on IMA. | ||
==References== | ==References== | ||
Revision as of 08:21, 30 July 2019
IMA(Instant Mash Agar)
IMA is sometimes also called Smash (after the brand name of the instant potato commonly used). Other brands of instant mashed potato can also be used to make up this medium.
Preparation
Per 100 mL:
- 2g Smash instant potato
- 2g agar
- 100mL tap water
Instructions
- Mix all the components together well in a 200mL Erlenmeyer flask with a bung, or other container suitable for autoclaving.
- Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
- Mix well and pour into sterile Petri dishes.
Uses
- Routine growth of Streptomyces sp.
- [Preparing spore stocks]]
Notes
- Because the agar and instant mashed potato are not very soluble, IMA should be made up in small 100 mL batches (do not attempt to make up 1 L and aliquot into flasks for autoclaving)
- ''Streptomyces scabies'' grows particularly well on IMA.
- Strains of ''Streptomyces coelicolor'' that fail to sporulate very well on SFM may sporulate better on IMA.
