ISP2: Difference between revisions
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ISP-2 is a rich medium that is routinely used for cultivating Streptmyces lydicus along with various other plant root associated strains. It contains malt extract and yeast extract, with dextrose as a carbon source; exact composition will vary between batches. | ISP-2 is a rich medium that is routinely used for cultivating Streptmyces lydicus along with various other plant root associated strains. It contains malt extract and yeast extract, with dextrose as a carbon source; exact composition will vary between batches. | ||
Many ''Streptomyces'' spp. grow well on | Many ''Streptomyces'' spp. grow well on ISP-2, but developmental and/or antibiotic production phenotypes may differ compared to growth on other media (e.g. [[SFM]]). | ||
==Preparation== | ==Preparation== | ||
Revision as of 16:05, 4 July 2019
International Streptomyces Project-2 (ISP-2)
ISP-2 is a rich medium that is routinely used for cultivating Streptmyces lydicus along with various other plant root associated strains. It contains malt extract and yeast extract, with dextrose as a carbon source; exact composition will vary between batches.
Many Streptomyces spp. grow well on ISP-2, but developmental and/or antibiotic production phenotypes may differ compared to growth on other media (e.g. SFM).
Preparation
Per Litre:
- 4g Yeast extract powder
- 10g Malt extract powder
- 4g Dextrose
- 20g Agar
- 1L dH2O
Instructions
- For solid medium, add 2g of Agar to each flask
- Add the rest of the components to a beaker with the dH2O
- Add 100ml to each flask
- Autoclave
Uses
- Streptomyces maintenance
- Spore titres
- Re-isolation experiments
- Selection for resistance to salt-sensitive antibiotics
Notes
For growth in liquid culture, ISP-2 can be made without the agar.
