ISP3

From ActinoBase
The printable version is no longer supported and may have rendering errors. Please update your browser bookmarks and please use the default browser print function instead.

International Streptomyces Project-3 Medium (ISP-3)

International Streptomyces Project Medium 3 (ISP-3) is a rich general use media. Alternatively called oatmeal agar.

Preparation

  • 20 g White Oats
  • 18 g Agar
  • 1 mL Trace salts solution
    • 0.1 g FeSO4 x 7H2O
    • 0.1 g MnCl2 x 4H2O
    • 0.1 g ZnSO4 x 7H2O
    • 100 mL dH2O
  • 1 L dH2O

Instructions

  1. Boil 20 g oats in 1L dH2O for 20 mins
  2. Filter oats through cheesecloth and top volume up to 1L
  3. Adjust pH to 7.2, add agar, and autoclave
  4. Add 1 mL Trace Salts solution

Uses

  • Inducing sporulation of environmental isolates
  • Screening for antibiotic production

Notes

When screening for antibiotic production, there can be significant differences between media prepared in-house and bought in media

References