Imaging GFP streptomyces on arabidopsis roots: Difference between revisions
From ActinoBase
Jake Newitt (talk | contribs) (Created page with "=Imaging GFP streptomyces on ''Arabidopsis'' roots= ''Streptomyces'' strains that contain pIJ8660/PermE* and are able to produce the eGFP protein encoded by it can be visuali...") |
Jake Newitt (talk | contribs) No edit summary |
||
Line 14: | Line 14: | ||
Adapted from | Adapted from | ||
Sarah F Worsley , Jake Newitt , Johannes Rassbach, Sibyl F D Batey, Neil A Holmes, J Colin Murrell, Barrie Wilkinson, Matthew I Hutchings - Streptomyces Endophytes Promote Host Health and Enhance Growth across Plant Species. | |||
Protocol adapted for ActinoBase by Jake T. Newitt, PhD student at the University of East Anglia. | Protocol adapted for ActinoBase by Jake T. Newitt, PhD student at the University of East Anglia. |
Revision as of 09:16, 28 March 2022
Imaging GFP streptomyces on Arabidopsis roots
Streptomyces strains that contain pIJ8660/PermE* and are able to produce the eGFP protein encoded by it can be visualised on the roots of Arabidopsis plants. This can be useful to ascertain whether the strain is able to colonise the surface or the interior of the root.
Preparation and reagents needed
Instructions
Notes
References
Adapted from
Sarah F Worsley , Jake Newitt , Johannes Rassbach, Sibyl F D Batey, Neil A Holmes, J Colin Murrell, Barrie Wilkinson, Matthew I Hutchings - Streptomyces Endophytes Promote Host Health and Enhance Growth across Plant Species.
Protocol adapted for ActinoBase by Jake T. Newitt, PhD student at the University of East Anglia.