Imaging GFP streptomyces on arabidopsis roots: Difference between revisions
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==Preparation and reagents needed== | ==Preparation and reagents needed== | ||
* | * ''Streptomyces'' modified with pIJ9660/PermE* - see [[Conjugation using ET12567/pUZ8002]] | ||
'''Instructions''' | '''Instructions''' |
Revision as of 09:21, 28 March 2022
Imaging GFP streptomyces on Arabidopsis roots
Streptomyces strains that contain pIJ8660/PermE* and are able to produce the eGFP protein encoded by it can be visualised on the roots of Arabidopsis plants. This can be useful to ascertain whether the strain is able to colonise the surface or the interior of the root.
Preparation and reagents needed
- Streptomyces modified with pIJ9660/PermE* - see Conjugation using ET12567/pUZ8002
Instructions
Notes
References
Adapted from
Sarah F Worsley , Jake Newitt , Johannes Rassbach, Sibyl F D Batey, Neil A Holmes, J Colin Murrell, Barrie Wilkinson, Matthew I Hutchings - Streptomyces Endophytes Promote Host Health and Enhance Growth across Plant Species.
Protocol adapted for ActinoBase by Jake T. Newitt, PhD student at the University of East Anglia.