Imaging GFP streptomyces on arabidopsis roots

Imaging GFP streptomyces on Arabidopsis roots

Streptomyces strains that contain pIJ8660/PermE* and are able to produce the eGFP protein encoded by it can be visualised on the roots of Arabidopsis plants. This can be useful to ascertain whether the strain is able to colonise the surface or the interior of the root.

Preparation and reagents needed

Instructions

References

Adapted from

Protocol adapted for ActinoBase by Jake T. Newitt, PhD student at the University of East Anglia.