Imaging GFP streptomyces on arabidopsis roots

Imaging GFP streptomyces on Arabidopsis roots

Streptomyces strains that contain pIJ8660/PermE* and are able to produce the eGFP protein encoded by it can be visualised on the roots of Arabidopsis plants. This can be useful to ascertain whether the strain is able to colonise the surface or the interior of the root.

Preparation and reagents needed

• Streptomyces modified with pIJ9660/PermE* - see Conjugation using ET12567/pUZ8002

Instructions

References

Adapted from

Sarah F Worsley , Jake Newitt , Johannes Rassbach, Sibyl F D Batey, Neil A Holmes, J Colin Murrell, Barrie Wilkinson, Matthew I Hutchings - Streptomyces Endophytes Promote Host Health and Enhance Growth across Plant Species.

Protocol adapted for ActinoBase by Jake T. Newitt, PhD student at the University of East Anglia.