Indicator compounds: Difference between revisions

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(Created page with "=Indicator Compounds= ==5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-Gal)== '''Uses''' *Detection of β-galactosidase activity on solid media *Bacterial two-hybrid a...")
 
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==5-Bromo-4-chloro-1'H'-indol-3-yl β-D-glucopyranosiduronic acid (X-Gluc)==
==5-Bromo-4-chloro-1H-indol-3-yl β-D-glucopyranosiduronic acid (X-Gluc)==


'''Uses'''
'''Uses'''
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'''Instructions for use'''
'''Instructions for use'''
#C
#C


==Notes==
==Notes==

Revision as of 15:11, 28 February 2020

Indicator Compounds

5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-Gal)

Uses

  • Detection of β-galactosidase activity on solid media
  • Bacterial two-hybrid analysis[1]

Instructions for use


5-Bromo-4-chloro-1H-indol-3-yl β-D-glucopyranosiduronic acid (X-Gluc)

Uses

  • Detection of β-glucuronidase activity on solid media
  • Screening for β-glucuronidase -positive or -negative candidates[1]

Instructions for use

  1. C

Notes

References

[1] Myronovskyi, M., Welle, E., Fedorenko, V., & Luzhetskyy, A. (2011). Beta-glucuronidase as a sensitive and versatile reporter in actinomycetes. Applied and environmental microbiology, 77(15), 5370–5383. https://doi.org/10.1128/AEM.00434-11