LB: Difference between revisions
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Sam Prudence (talk | contribs) (Created page with "=LB= Known as Lennox Broth or Lysogeny Broth ==For 1 Litre:== *5g Yeast Extract *10g NaCl *10g Tryptone *Up to 1L dH2O '''Instructions''' #Add 5g Yeast Extract, 10g NaCl and...") |
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#Autoclave | #Autoclave | ||
Note: | |||
When using Hygromycin, for example when cloning into [[pSS170]], use the -NaCl (no salt) version of LB. | When using salt sensitive antibiotics like Hygromycin, for example when cloning into [[pSS170]], use the -NaCl (no salt) version of LB below. | ||
==-NaCl LB== | ==-NaCl LB== | ||
==For 1 Litre:== | ===For 1 Litre:=== | ||
*5g Yeast Extract | *5g Yeast Extract | ||
*10g NaCl | *10g NaCl | ||
*10g Tryptone | *10g Tryptone | ||
*Up to 1L dH2O | *Up to 1L dH2O | ||
Revision as of 19:56, 20 June 2019
LB
Known as Lennox Broth or Lysogeny Broth
For 1 Litre:
- 5g Yeast Extract
- 10g NaCl
- 10g Tryptone
- Up to 1L dH2O
Instructions
- Add 5g Yeast Extract, 10g NaCl and 10g Tryptone to a beaker
- Add 800ml dH2O and stir until all solids are fully suspended
- Make up to 1L with dH2O
- For solid media, add 4g agar per 200ml LB
- Autoclave
Note: When using salt sensitive antibiotics like Hygromycin, for example when cloning into pSS170, use the -NaCl (no salt) version of LB below.
-NaCl LB
For 1 Litre:
- 5g Yeast Extract
- 10g NaCl
- 10g Tryptone
- Up to 1L dH2O
