LB

LB

Known as Lennox Broth or Lysogeny Broth

For 1 Litre:

• 5g Yeast Extract
• 10g NaCl
• 10g Tryptone
• Up to 1L dH2O

Instructions

1. Add 5g Yeast Extract, 10g NaCl and 10g Tryptone to a beaker
2. Add 800ml dH2O and stir until all solids are fully suspended
3. Make up to 1L with dH2O
4. For solid media, add 4g agar per 200ml LB
5. Autoclave

Note: When using salt sensitive antibiotics like Hygromycin, for example when cloning into pSS170, use the -NaCl (no salt) version of LB below.

-NaCl LB

For 1 Litre:

• 5g Yeast Extract
• 10g NaCl
• 10g Tryptone
• Up to 1L dH2O