Lambda DNA/HindIII Ladder

Lambda HindIII Marker Preparation

• Lambda (λ) DNA (500 µg/ml): 100µl
• 10x Reaction Buffer: 45 µl
• HindIII or PstI(10U/µl): 5µl
• ddWater: 295µl

Preparation:

1. Incubate 90 minutes 37°C
2. Add 5µl HindIII or PstI
3. Incubate 90 minutes 37°
4. Incubate 10 minutes, 75°C
5. Store at –20°C

Add 6x loading dye before loading into gel

Size and Concentration of Bands

Size of Bands in bp (λ HindIII)	ng in 5µl
(27491)	-
(23130)	240
9416	97
6556	68
(4361)	44
2322	24
2027	21
561	6
125	-