MC agar: Difference between revisions
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=MC agar= | =MC agar= | ||
This media is used to screen Streptomyces strains for extracellular protease production and the resulting degradation of the milk powder components on the plate, which can be detected as protein degradation "halos" (visually resemble zone of inhibtions that one would observe with bioassays). This recipe was adjusted for Streptomyces clavuligerus. | This media is used to screen ''Streptomyces'' strains for extracellular protease production and the resulting degradation of the milk powder components on the plate, which can be detected as protein degradation "halos" (visually resemble zone of inhibtions that one would observe with bioassays). This recipe was adjusted for Streptomyces clavuligerus. | ||
==Preparation== | ==Preparation== | ||
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==Uses== | ==Uses== | ||
After heat-activation and pre-germination of Streptomyces spores, they can then be spotted (~5µl)onto the agar for sharp edges of the colonies and the resulting protease halos. | After heat-activation and pre-germination of ''Streptomyces'' spores, they can then be spotted (~5µl)onto the agar for sharp edges of the colonies and the resulting protease halos. | ||
==References== | ==References== |
Revision as of 15:10, 26 July 2019
MC agar
This media is used to screen Streptomyces strains for extracellular protease production and the resulting degradation of the milk powder components on the plate, which can be detected as protein degradation "halos" (visually resemble zone of inhibtions that one would observe with bioassays). This recipe was adjusted for Streptomyces clavuligerus.
Preparation
Per litre:
- 50 g Dry milk powder
- 5 g Tryptone
- 2.5 g Yeast Extract
- 12.5 g Agar
- 5 g Glucose (add glucose from stock solution after autoclaving)
- 1 L dH2O
Instructions
- Dissolve all components (but glucose and agar) in 0.9 L dH2O
- Adjust pH to 6.8 - 7.0
- Add and dissolve agar
- Autoclave
- Add 100 ml of a filtersterilised 50% glucose solution
Note
- The milk powder in the media will curdle, mix gently by swirling bottle prior to pouring plates
- if alternative carbon sources are added to the media, adjust number of carbon atoms accordingly,
Example: add 10 g/L of glycerol (C3), or 2.5 g/L of maltose (C12)
Uses
After heat-activation and pre-germination of Streptomyces spores, they can then be spotted (~5µl)onto the agar for sharp edges of the colonies and the resulting protease halos.