MYM: Difference between revisions
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(Created page with "=MYM Medium= ==For 1 Litre:== *4g Maltose *4g Yeast Extract Oxoid *10g Malt Extract *500ml Tap Water *500ml dH2O '''Instructions''' #Dissolve all ingredients in...") |
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#Dissolve all ingredients in 500ml tap water & 250ml dH2O | #Dissolve all ingredients in 500ml tap water & 250ml dH2O | ||
#pH to 7.2-7.3 with NaOH | #pH to 7.2-7.3 with NaOH | ||
#add dH2O up to 1L | #add dH2O up to 1L | ||
#For solid medium add add 2g agar & 100mL liquid MYM to each flask | #For solid medium add add 2g agar & 100mL liquid MYM to each flask | ||
#Autoclave, expect a precipitate to form after autclaving. | #Autoclave, expect a precipitate to form after autclaving. | ||
Note: If using for growth measurements in liquid medium allow the precipitate to settle overnight before pipetting medium into flasks, taking from the top to avoid the precipitate. | Note: If using for growth measurements in liquid medium allow the precipitate to settle overnight before pipetting medium into flasks, taking from the top to avoid the precipitate. | ||
Revision as of 16:15, 6 May 2019
MYM Medium
For 1 Litre:
- 4g Maltose
- 4g Yeast Extract Oxoid
- 10g Malt Extract
- 500ml Tap Water
- 500ml dH2O
Instructions
- Dissolve all ingredients in 500ml tap water & 250ml dH2O
- pH to 7.2-7.3 with NaOH
- add dH2O up to 1L
- For solid medium add add 2g agar & 100mL liquid MYM to each flask
- Autoclave, expect a precipitate to form after autclaving.
Note: If using for growth measurements in liquid medium allow the precipitate to settle overnight before pipetting medium into flasks, taking from the top to avoid the precipitate.
