MYM: Difference between revisions
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#For solid medium add add 2g agar & 100mL liquid MYM to each flask | #For solid medium add add 2g agar & 100mL liquid MYM to each flask | ||
#Autoclave, expect a precipitate to form afterward. | #Autoclave, expect a precipitate to form afterward. | ||
#To encourage [[ | #To encourage ''[[Streptomyces venezuelae]]'' sporulation, add [[trace elements]] at a 1:500 dilution. | ||
Note: If using for growth measurements in liquid medium, it may be best to allow the precipitate to settle overnight before pipetting medium into growth vessels, taking from the top to avoid the precipitate. | Note: If using for growth measurements in liquid medium, it may be best to allow the precipitate to settle overnight before pipetting medium into growth vessels, taking from the top to avoid the precipitate. | ||
Revision as of 16:24, 6 May 2019
MYM Medium
For 1 Litre:
- 4g Maltose
- 4g Yeast Extract Oxoid
- 10g Malt Extract
- 500ml Tap Water
- 500ml dH2O
Instructions
- Dissolve all ingredients in 500ml tap water & 250ml dH2O
- pH to 7.2-7.3 with NaOH
- add dH2O up to 1L
- For solid medium add add 2g agar & 100mL liquid MYM to each flask
- Autoclave, expect a precipitate to form afterward.
- To encourage Streptomyces venezuelae sporulation, add trace elements at a 1:500 dilution.
Note: If using for growth measurements in liquid medium, it may be best to allow the precipitate to settle overnight before pipetting medium into growth vessels, taking from the top to avoid the precipitate.
