MtrA methods are here: Difference between revisions
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Som N, Devine R, Chandra G, Martins C, Wilkinson B and Hutchings MI (2023). MtrA regulates the expression of developmental genes, including BldM and WhiI, in Streptomyces venezuelae. Manuscript in preparation. | This page contains methods used in the following study: | ||
<strong>Som N, Devine R, Chandra G, Martins C, Wilkinson B and Hutchings MI (2023). MtrA regulates the expression of developmental genes, including BldM and WhiI, in Streptomyces venezuelae. Manuscript in preparation.</strong> | |||
<u>Microbiology methods (links to sub pages)</u> | |||
[[ SFM ]] agar recipe | |||
[[ General_Growth_and_Maintenance_of_Streptomyces_sp ]] | |||
[https://www.youtube.com/watch?v=aSuCRp0ilIo <em>Streptomyces</em> spore preparation] | |||
[[ Conjugation_using_ET12567/pUZ8002 ]] | |||
The [[PCRISPomyces-2]] plasmid for CRISPR/Cas9 mediated genome editing | |||
[[ CRISPR/Cas9-mediated_genome_editing ]] | |||
<u>ReDCaT surface plasmon resonance</u> | |||
This technique is described in this publication [[https://pubmed.ncbi.nlm.nih.gov/33877608/]]. Methods are given in detail below. | |||
Latest revision as of 16:17, 12 September 2023
This page contains methods used in the following study:
Som N, Devine R, Chandra G, Martins C, Wilkinson B and Hutchings MI (2023). MtrA regulates the expression of developmental genes, including BldM and WhiI, in Streptomyces venezuelae. Manuscript in preparation.
Microbiology methods (links to sub pages)
SFM agar recipe
General_Growth_and_Maintenance_of_Streptomyces_sp
Streptomyces spore preparation
Conjugation_using_ET12567/pUZ8002
The PCRISPomyces-2 plasmid for CRISPR/Cas9 mediated genome editing
CRISPR/Cas9-mediated_genome_editing
ReDCaT surface plasmon resonance
This technique is described in this publication [[1]]. Methods are given in detail below.
