MtrA methods are here

This page contains methods used in the following study:

Som N, Devine R, Chandra G, Martins C, Wilkinson B and Hutchings MI (2023). MtrA regulates the expression of developmental genes, including BldM and WhiI, in Streptomyces venezuelae. Manuscript in preparation.

Microbiology methods (links to sub pages)

SFM agar recipe

General_Growth_and_Maintenance_of_Streptomyces_sp

Streptomyces spore preparation

Conjugation_using_ET12567/pUZ8002

The PCRISPomyces-2 plasmid for CRISPR/Cas9 mediated genome editing

CRISPR/Cas9-mediated_genome_editing

ReDCaT surface plasmon resonance

This technique is described in this publication [[1]]. Methods are given in detail below.