PCAP03: Difference between revisions
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*ΦC31 integrative site & integrase | *ΦC31 integrative site & integrase | ||
*URA3 | *URA3 | ||
*NeoR/KanR | |||
==History== | ==History== | ||
Revision as of 10:42, 13 August 2019
Use
The pCAP03 plasmid has been successfully used to create capture vectors that can be used for TAR cloning of biosynthetic gene clusters. Capture arms can be cloned within the restriction enzymes sites, and the plasmid can be selected for with kanamycin. pCAP03 contains a URA3 gene that allows for selection against non-homologous end joining in the presence of 5-fluoroorotic acid (5-FOA).
Features
- Apramycin resistance cassette (acc(3)IV)
- ΦC31 integrative site & integrase
- URA3
- NeoR/KanR
History
The plasmid was developed by Bradley Moore's lab as an upgraded version of pCAP01.
Map
Sequence links
See AddGene link (here) to access the sequence.
References
The paper reporting pCAP03 is here
