PCAP03: Difference between revisions
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==Use== | ==Use== | ||
The pCAP03 plasmid has been successfully used to create capture vectors that can be used for [[TAR cloning]] of biosynthetic gene clusters. Capture arms can be cloned within the restriction enzymes sites. The plasmid contains elements that allow transfer between <em>E. coli</em>, | The pCAP03 plasmid has been successfully used to create capture vectors that can be used for [[TAR cloning]] of biosynthetic gene clusters. Capture arms can be cloned within the restriction enzymes sites. The plasmid contains elements that allow transfer between <em>E. coli</em>, actinobacteria and yeast cells. | ||
==Features== | ==Features== | ||
Revision as of 11:05, 13 August 2019
Use
The pCAP03 plasmid has been successfully used to create capture vectors that can be used for TAR cloning of biosynthetic gene clusters. Capture arms can be cloned within the restriction enzymes sites. The plasmid contains elements that allow transfer between E. coli, actinobacteria and yeast cells.
Features
- Apramycin resistance cassette (acc(3)IV)
- ΦC31 integrative site & integrase
- URA3
- Neomycin and kanamycin resistance
- pUC ori for maintenance in E. coli
History
The plasmid was developed by Bradley Moore's lab as an upgraded version of pCAP01.
Map
Sequence links
See AddGene link (here) to access the sequence.
References
The paper reporting pCAP03 is here
