PCAP03: Difference between revisions
| Line 10: | Line 10: | ||
*Neomycin and kanamycin resistance | *Neomycin and kanamycin resistance | ||
*pUC <em>ori</em> for maintenance in <em>E. coli</em> | *pUC <em>ori</em> for maintenance in <em>E. coli</em> | ||
*Yeast elements: autonomous replication sequence ARSH4, centromere CEN6, selection marker TRP1 | |||
==History== | ==History== | ||
Revision as of 11:25, 13 August 2019
Use
The pCAP03 plasmid has been successfully used to create capture vectors that can be used for TAR cloning of biosynthetic gene clusters. Capture arms can be cloned within the restriction enzymes sites. The plasmid contains elements that allow transfer between E. coli, actinobacteria and yeast cells.
Features
- Apramycin resistance cassette (acc(3)IV)
- ΦC31 integrative site & integrase
- URA3
- Neomycin and kanamycin resistance
- pUC ori for maintenance in E. coli
- Yeast elements: autonomous replication sequence ARSH4, centromere CEN6, selection marker TRP1
History
The plasmid was developed by Bradley Moore's lab as an upgraded version of pCAP011.
Map
Sequence links
See AddGene link (here) to access the sequence.
References
The paper reporting pCAP03 is here
1. Yamanaka K., Reynolds K.A., Kersten R.D., Ryan K.S., Gonzalez D.J., Nizet V., Dorrestein P.C., Moore B.S. (2014) Direct cloning and refactoring of a silent lipopeptide biosynthetic gene cluster yields the antibiotic taromycin A. Proceedings of the National Academy of Sciences of the United States of America 111(5):1957-62. doi: 10.1073/pnas.1319584111
