PCAP03: Difference between revisions
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{{DISPLAYTITLE: pCAP03}} | {{DISPLAYTITLE: pCAP03}} | ||
= | ==Use== | ||
The pCAP03 plasmid has been successfully used to create capture vectors that can be used for TAR cloning of biosynthetic gene clusters. | The pCAP03 plasmid has been successfully used to create capture vectors that can be used for TAR cloning of biosynthetic gene clusters. | ||
== | ==Features== | ||
*Apramycin resistance cassette (acc(3)IV). | |||
Capture arms can be cloned within the restriction enzymes sites, and the plasmid can be selected for with kanamycin. | Capture arms can be cloned within the restriction enzymes sites, and the plasmid can be selected for with kanamycin. | ||
pCAP03 contains a URA3 gene that allows for selection against non-homologous end joining in the presence of 5-fluoroorotic acid (5-FOA). | pCAP03 contains a URA3 gene that allows for selection against non-homologous end joining in the presence of 5-fluoroorotic acid (5-FOA). | ||
== | ==History== | ||
The plasmid | The plasmid was developed by [http://scrippsscholars.ucsd.edu/bsmoore/pages/moore-lab-research Bradley Moore's] lab as an upgraded version of pCAP01. | ||
==Map== | |||
==Sequence links== | |||
See AddGene link ([https://www.addgene.org/69862/ here]) to access the sequence. | |||
==References== | ==References== | ||
The paper reporting pCAP03 is [https://pubs.acs.org/doi/10.1021/acschembio.5b00658 here] | The paper reporting pCAP03 is [https://pubs.acs.org/doi/10.1021/acschembio.5b00658 here] | ||
Revision as of 16:43, 8 August 2019
Use
The pCAP03 plasmid has been successfully used to create capture vectors that can be used for TAR cloning of biosynthetic gene clusters.
Features
- Apramycin resistance cassette (acc(3)IV).
Capture arms can be cloned within the restriction enzymes sites, and the plasmid can be selected for with kanamycin. pCAP03 contains a URA3 gene that allows for selection against non-homologous end joining in the presence of 5-fluoroorotic acid (5-FOA).
History
The plasmid was developed by Bradley Moore's lab as an upgraded version of pCAP01.
Map
Sequence links
See AddGene link (here) to access the sequence.
References
The paper reporting pCAP03 is here
