pCAP03

Use

The pCAP03 plasmid has been successfully used to create capture vectors that can be used for TAR cloning of biosynthetic gene clusters. Capture arms can be cloned within the restriction enzymes sites, and the plasmid can be selected for with kanamycin. pCAP03 contains a URA3 gene that allows for selection against non-homologous end joining in the presence of 5-fluoroorotic acid (5-FOA).

Features

• Apramycin resistance cassette (acc(3)IV)
• ΦC31 integrative site & integrase
• URA3

History

The plasmid was developed by Bradley Moore's lab as an upgraded version of pCAP01.

Map

Sequence links

See AddGene link (here) to access the sequence.

References

The paper reporting pCAP03 is here