PCRISPomyces-2: Difference between revisions
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Click here for the [[CRISPR]] protocol used by Matt Hutchings lab (courtesy of Rebecca Devine) - [http://www.hutchingslab.uk/downloads/pCRISPOmyces2.pdf or download the PDF]. | Click here for the [[CRISPR]] protocol used by Matt Hutchings lab (courtesy of Rebecca Devine) - [http://www.hutchingslab.uk/downloads/pCRISPOmyces2.pdf or download the PDF]. | ||
The plasmid was made by | The plasmid was made by [http://faculty.scs.illinois.edu/~zhaogrp/index.html Huimin Zhao's] group and is available free from AddGene under MTA: [https://www.addgene.org/61737/ click here] | ||
[[file:CRISPR.png]] | [[file:CRISPR.png]] | ||
Revision as of 08:17, 24 July 2019
This plasmid can be used for precise CRISPR/Cas9-mediated genome engineering of Streptomyces strains. To our knowledge it has been successfully used in S. albidoflavus, S. coelicolor, S. formicae, S. lividans and S. venezuelae. Mutations have ranged from 1-100kbp deletions, precise codon changes to alter amino acids and insertions to add Flag-tags to proteins encoded at their native loci.
Click here for the CRISPR protocol used by Matt Hutchings lab (courtesy of Rebecca Devine) - or download the PDF.
The plasmid was made by Huimin Zhao's group and is available free from AddGene under MTA: click here
