PCRISPomyces-2

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Revision as of 20:16, 18 July 2019 by Matth (talk | contribs)

This plasmid can be used for precise CRISPR/Cas9-mediated genome engineering of Streptomyces strains. To our knowledge it has been successfully used in S. albidoflavus, S. coelicolor, S. formicae, S. lividans and S. venezuelae. Mutations have ranged from 1-100kbp deletions, precise codon changes to alter amino acids and insertions to add Flag-tags to proteins encoded at their native loci.

The paper describing pCRISPomyces2

The protocol from Matt Hutchings lab - download as a PDF.

The plasmid is available from AddGene under MTA: click here

CRISPR.png