PKC1132: Difference between revisions
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==Use== | ==Use== | ||
pKC1132 is a suicide vector that can be used for homologous recombination experiments such as gene deletions and disruptions. It is a conjugative, non-integrative plasmid. | pKC1132 is a suicide vector that can be used for cloning and homologous recombination experiments such as gene deletions and disruptions. It is a conjugative, non-integrative plasmid that can be used to make constructs in <em>E. coli</em> that can be transferred by conjugation into <em>Streptomyces</em> species. | ||
==Features== | ==Features== |
Revision as of 14:09, 13 August 2019
Use
pKC1132 is a suicide vector that can be used for cloning and homologous recombination experiments such as gene deletions and disruptions. It is a conjugative, non-integrative plasmid that can be used to make constructs in E. coli that can be transferred by conjugation into Streptomyces species.
Features
- Apramycin resistance
- Origin of transfer oriT from RK2