PKC1132: Difference between revisions
From ActinoBase
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*Apramycin resistance | *Apramycin resistance | ||
*Origin of transfer <em>oriT</em> from RK2 | *Origin of transfer <em>oriT</em> from RK2 | ||
*Multiple cloning site within <em>lacZa</em> to allow for detection of recombinant clones | |||
==History== | ==History== | ||
Revision as of 14:13, 13 August 2019
Use
pKC1132 is a suicide vector that can be used for cloning and homologous recombination experiments such as gene deletions and disruptions. It is a conjugative, non-integrative plasmid that can be used to make constructs in E. coli that can be transferred by conjugation into Streptomyces species.
Features
- Apramycin resistance
- Origin of transfer oriT from RK2
- Multiple cloning site within lacZa to allow for detection of recombinant clones
History
Map
