PKC1132: Difference between revisions
From ActinoBase
| Line 21: | Line 21: | ||
==References== | ==References== | ||
Bierman, M., Logan, R., O'Brien, K., Seno, E.T., Nagaraja Rao, R. and Schoner, B.E. (1992) Plasmid cloning vectors for the conjugal transfer of DNA from <em>Escherichia coli</em> to <em>Streptomyces</em> spp. <em>Gene</em> 116, 43-49 | |||
Revision as of 14:18, 13 August 2019
Use
pKC1132 is a suicide vector that can be used for cloning and homologous recombination experiments such as gene deletions and disruptions. It is a conjugative, non-integrative plasmid that can be used to make constructs in E. coli that can be transferred by conjugation into Streptomyces species.
Features
- Apramycin resistance
- Origin of transfer oriT from RK2
- Multiple cloning site within lacZa to allow for detection of recombinant clones
History
pKC1132 is a derivative of pOJ260 with a deletion between the KpnI to SpeI sites.1
Map
Sequence links
References
Bierman, M., Logan, R., O'Brien, K., Seno, E.T., Nagaraja Rao, R. and Schoner, B.E. (1992) Plasmid cloning vectors for the conjugal transfer of DNA from Escherichia coli to Streptomyces spp. Gene 116, 43-49
