PKC1132: Difference between revisions
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==Use== | ==Use== | ||
pKC1132 is a suicide vector that can be used for homologous recombination experiments such as gene deletions and disruptions. It is a conjugative, non-integrative plasmid. | pKC1132 is a suicide vector that can be used for cloning and homologous recombination experiments such as gene deletions and disruptions. It is a conjugative, non-integrative plasmid that can be used to make constructs in <em>E. coli</em> that can be transferred by conjugation into <em>Streptomyces</em> species.<sup>1</sup> It also contains a <em>lacZa</em> gene to allow for blue/white screening of recombinant clones in <em>E. coli</em>. | ||
==Features== | ==Features== | ||
*Apramycin resistance | |||
*Origin of transfer <em>oriT</em> from RK2 | |||
*Multiple cloning site within <em>lacZa</em> to allow for screening of recombinant clones | |||
==History== | |||
pKC1132 is a derivative of pOJ260 with a deletion between the <em>Kpn</em>I to <em>Spe</em>I sites.<sup>1</sup> | |||
==Map== | ==Map== | ||
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==References== | ==References== | ||
1. Bierman, M., Logan, R., O'Brien, K., Seno, E.T., Nagaraja Rao, R. and Schoner, B.E. (1992) Plasmid cloning vectors for the conjugal transfer of DNA from <em>Escherichia coli</em> to <em>Streptomyces</em> spp. <em>Gene</em> 116, 43-49 | |||
Latest revision as of 14:24, 13 August 2019
Use
pKC1132 is a suicide vector that can be used for cloning and homologous recombination experiments such as gene deletions and disruptions. It is a conjugative, non-integrative plasmid that can be used to make constructs in E. coli that can be transferred by conjugation into Streptomyces species.1 It also contains a lacZa gene to allow for blue/white screening of recombinant clones in E. coli.
Features
- Apramycin resistance
- Origin of transfer oriT from RK2
- Multiple cloning site within lacZa to allow for screening of recombinant clones
History
pKC1132 is a derivative of pOJ260 with a deletion between the KpnI to SpeI sites.1
Map
Sequence links
References
1. Bierman, M., Logan, R., O'Brien, K., Seno, E.T., Nagaraja Rao, R. and Schoner, B.E. (1992) Plasmid cloning vectors for the conjugal transfer of DNA from Escherichia coli to Streptomyces spp. Gene 116, 43-49
