pKC1132

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Revision as of 14:13, 13 August 2019 by Alicia (talk | contribs) (→‎Features)


Use

pKC1132 is a suicide vector that can be used for cloning and homologous recombination experiments such as gene deletions and disruptions. It is a conjugative, non-integrative plasmid that can be used to make constructs in E. coli that can be transferred by conjugation into Streptomyces species.

Features

  • Apramycin resistance
  • Origin of transfer oriT from RK2
  • Multiple cloning site within lacZa to allow for detection of recombinant clones

History

Map

PKC1132 Map.png

Sequence links

References