PMS82: Difference between revisions

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[file:pMS82.png]
{{DISPLAYTITLE: pMS82}}


Integrating vector derived from the φBT1 attP-int locus and carrying the hygromycin resistance cassette. Described [https://jb.asm.org/content/185/17/5320.long here]
 
==Use==
 
This integration vector allows for conjugal transfer of DNA into the φBT1 phage attachment site of <Em>Streptomyces</Em> genomes from <Em>E. coli</Em>. This vector is derived from the pSET152 backbone. It contains the <EM>oriT</Em> from <Em>Streptomyces</Em> as well as the hygromycin resistance cassette.
 
This vector can be manipulated to contain genes of interest for the expression of homologous proteins, photoproteins, natural products etc.
 
==Features==
*Hygromycin resistance cassette
*φBT1 integration site & integrase
 
==History==
 
==Map==
[[File:pMS82.png]]
 
 
==Sequence links==
 
==References==
===Needs formatting!!===
[https://jb.asm.org/content/185/17/5320.long here]

Latest revision as of 13:32, 25 October 2019


Use

This integration vector allows for conjugal transfer of DNA into the φBT1 phage attachment site of Streptomyces genomes from E. coli. This vector is derived from the pSET152 backbone. It contains the oriT from Streptomyces as well as the hygromycin resistance cassette.

This vector can be manipulated to contain genes of interest for the expression of homologous proteins, photoproteins, natural products etc.

Features

  • Hygromycin resistance cassette
  • φBT1 integration site & integrase

History

Map

PMS82.png


Sequence links

References

Needs formatting!!

here