PRT802

Use

This vector is for general use in Streptomyces. For example, producing marked strains for re-isolation experiments, or gene expression, over expression, or complementation.

Features

• Kanamycin resistance gene
• ΦBT1 phage integration site and integrase
• Multiple cloning site

History

Backbone vector pSET152 was modified to replace the ΦC31 integrative machinery with the ΦBT1 integrative machinery by Maggie Smiths group, published in 2003¹.

Map

Sequence links

addgene page Click "sequence" to download .gbk file.

References

1. Gregory, M.A., Till, R., Smith, M.C.M. (2003). Integration Site for Streptomyces Phage ΦBT1 and Development of

Site-Specific Integrating Vectors. Journal of Bacteriology, 185(17), pp. 5320-5323. DOI: 10.1128/JB.185.17.5320–5323.2003