pRT802
From ActinoBase
pRT802
Use
This vector is for general use in Streptomyces. For example, producing marked strains for re-isolation experiments, or gene expression, over expression, or complementation.
Features
- Kanamycin resistance gene
- ΦBT1 phage integration site and integrase
- Multiple cloning site
History
Backbone vector pSET152 was modified to replace the ΦC31 integrative machinery with the ΦBT1 integrative machinery by Maggie Smiths group, published in 20031.
Map
Sequence links
addgene page Click "sequence" to download .gbk file.
References
- Gregory, M.A., Till, R., Smith, M.C.M. (2003). Integration Site for Streptomyces Phage ΦBT1 and Development of
Site-Specific Integrating Vectors. Journal of Bacteriology, 185(17), pp. 5320-5323. DOI: 10.1128/JB.185.17.5320–5323.2003