pSS170

Use

pSS170 is used for introducing genes to Streptomyces. Because this plasmid is lacking a promoter upstream of the multiple cloning site (MCS), the gene of interest is often cloned in with its native promoter, in order to complement a knockout mutant

Features

Streptomyces phage phiBT1 integration site. Hygromycin resistance gene, oriT, ColEi origin of replication

History

Derived from pMS82 - https://www.addgene.org/68110/

References

pMS82 background vector - Gregory, M.A., Till, R. and Smith, M.C., 2003. Integration site for Streptomyces phage φBT1 and development of site-specific integrating vectors. Journal of bacteriology, 185(17), pp.5320-5323.