Plasmids: Difference between revisions
No edit summary |
|||
(One intermediate revision by one other user not shown) | |||
Line 10: | Line 10: | ||
[[pCRISPomyces-2]] | [[pCRISPomyces-2]] | ||
[[pCRISPR-Cas9]] | |||
[[pCRISPR-dCas9]] | |||
[[pESAC13]] | [[pESAC13]] | ||
Line 22: | Line 26: | ||
[[pIJ12742]] | [[pIJ12742]] | ||
[[pIJ6902]] | |||
[[pKC1132]] | [[pKC1132]] | ||
Line 34: | Line 40: | ||
[[pSS170]] | [[pSS170]] | ||
[[pTOYAMAcos]] | |||
[[pTYM18]] | |||
[[pTYM19]] | |||
[[Supercos1]] | [[Supercos1]] | ||
Line 40: | Line 52: | ||
[[pGM190]] | [[pGM190]] | ||
==Plasmids used for PCR targeting (Redirect)== | ==Plasmids used for PCR targeting (Redirect)== |
Revision as of 16:18, 18 May 2021
Various plasmids have been developed for use in Streptomyces (most will also contain an origin of replication that functions in Escherichia coli, for cloning, general maintenance, and intergeneric conjugation into Streptomyces). Many of the plasmids commonly used for Streptomyces work are integrative: they insert into a specific site on the Streptomyces chromosome using a site-specific phage recombinase.
The following is an alphabetical list of a number of useful vectors - see also Overview of Streptomyces vectors.
Plasmids used in Streptomyces
Plasmids used for PCR targeting (Redirect)
Redirect protocol and template information
Plasmids used for heterologous protein expression in E. coli
The following plasmids are frequently used to express proteins heterologously in E. coli, e.g. for two-hybrid analysis or protein purification.
Vectors for Bacterial two-hybrid experiments pKT25/pKNT25 | pUT18/pUT18C