Plasmids: Difference between revisions
Sam Prudence (talk | contribs) No edit summary |
|||
(6 intermediate revisions by 4 users not shown) | |||
Line 1: | Line 1: | ||
[[File:Actinobase-icons-trans_Plasmids.png|150px|Plasmids|link=Plasmids]] | [[File:Actinobase-icons-trans_Plasmids.png|150px|Plasmids|link=Plasmids]] | ||
Various plasmids have been developed for use in <em>Streptomyces</em> (most will also contain an origin of replication that functions in <em>Escherichia coli</em>, for cloning, general maintenance, and [[ | Various plasmids have been developed for use in <em>Streptomyces</em> (most will also contain an origin of replication that functions in <em>Escherichia coli</em>, for cloning, general maintenance, and [[intergeneric conjugation into Streptomyces]]). Many of the plasmids commonly used for <em>Streptomyces</em> work are '''integrative''': they insert into a specific site on the <em>Streptomyces</em> chromosome using a [[site-specific phage recombinase]]. | ||
The following is an alphabetical list of a number of useful vectors - see also [[Overview of Streptomyces vectors]]. | The following is an alphabetical list of a number of useful vectors - see also [[Overview of Streptomyces vectors]]. | ||
Line 9: | Line 9: | ||
[[pCAP03]] | [[pCAP03]] | ||
[[ | [[pCRISPomyces-2]] | ||
[[pCRISPR-Cas9]] | |||
[[pCRISPR-dCas9]] | |||
[[pESAC13]] | [[pESAC13]] | ||
Line 22: | Line 26: | ||
[[pIJ12742]] | [[pIJ12742]] | ||
[[pIJ6902]] | |||
[[pKC1132]] | [[pKC1132]] | ||
Line 34: | Line 40: | ||
[[pSS170]] | [[pSS170]] | ||
[[pTOYAMAcos]] | |||
[[pTYM18]] | |||
[[pTYM19]] | |||
[[Supercos1]] | [[Supercos1]] | ||
Line 41: | Line 53: | ||
[[pGM190]] | [[pGM190]] | ||
==Plasmids used for PCR targeting (Redirect)== | |||
[http://streptomyces.org.uk/redirect/index.html Redirect protocol and template information] | |||
[[pIJ773]] | [[pIJ773]] | ||
Line 53: | Line 66: | ||
[[pET28a]] | [[pET28a]] | ||
[[pKNT25 | Vectors for Bacterial two-hybrid experiments | ||
[[pKT25/pKNT25]] | [[pUT18/pUT18C]] |
Revision as of 16:18, 18 May 2021
Various plasmids have been developed for use in Streptomyces (most will also contain an origin of replication that functions in Escherichia coli, for cloning, general maintenance, and intergeneric conjugation into Streptomyces). Many of the plasmids commonly used for Streptomyces work are integrative: they insert into a specific site on the Streptomyces chromosome using a site-specific phage recombinase.
The following is an alphabetical list of a number of useful vectors - see also Overview of Streptomyces vectors.
Plasmids used in Streptomyces
Plasmids used for PCR targeting (Redirect)
Redirect protocol and template information
Plasmids used for heterologous protein expression in E. coli
The following plasmids are frequently used to express proteins heterologously in E. coli, e.g. for two-hybrid analysis or protein purification.
Vectors for Bacterial two-hybrid experiments pKT25/pKNT25 | pUT18/pUT18C