Plasmids: Difference between revisions
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Various plasmids have been developed for use in <em>Streptomyces</em> (most will also contain an origin of replication that functions in <em>Escherichia coli</em>, for cloning, general maintenance, and [[intergeneric conjugation into Streptomyces]]). Many of the plasmids commonly used for <em>Streptomyces</em> work are '''integrative''': they insert into a specific site on the <em>Streptomyces</em> chromosome using a [[site-specific phage recombinase]]. | Various plasmids have been developed for use in <em>Streptomyces</em> (most will also contain an origin of replication that functions in <em>Escherichia coli</em>, for cloning, general maintenance, and [[intergeneric conjugation into Streptomyces]]). Many of the plasmids commonly used for <em>Streptomyces</em> work are '''integrative''': they insert into a specific site on the <em>Streptomyces</em> chromosome using a [[site-specific phage recombinase]]. | ||
The following is an alphabetical list of a number of useful vectors - see also [[Overview of Streptomyces vectors]]. | The following is an alphabetical list of a number of useful vectors - see also [[Overview of Streptomyces vectors]]. Some of these vectors are available under MTA from [https://www.addgene.org/ Addgene]. Many others are also available under MTA by request at [mailto:jic.strepstrains@jic.ac.uk|JIC StrepStrains]. | ||
==Plasmids used in <em>Streptomyces</em>== | ==Plasmids used in <em>Streptomyces</em>== | ||
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[[pCRISPomyces-2]] | [[pCRISPomyces-2]] | ||
[[pCRISPR-Cas9]] | |||
[[pCRISPR-dCas9]] | |||
[[pESAC13]] | [[pESAC13]] | ||
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[[pSS170]] | [[pSS170]] | ||
[[pTOYAMAcos]] | |||
[[pTYM18]] | |||
[[pTYM19]] | |||
[[Supercos1]] | [[Supercos1]] | ||
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[[pGM190]] | [[pGM190]] | ||
==Plasmids used for PCR targeting (Redirect)== | ==Plasmids used for PCR targeting (Redirect)== |
Latest revision as of 17:41, 3 March 2022
Various plasmids have been developed for use in Streptomyces (most will also contain an origin of replication that functions in Escherichia coli, for cloning, general maintenance, and intergeneric conjugation into Streptomyces). Many of the plasmids commonly used for Streptomyces work are integrative: they insert into a specific site on the Streptomyces chromosome using a site-specific phage recombinase.
The following is an alphabetical list of a number of useful vectors - see also Overview of Streptomyces vectors. Some of these vectors are available under MTA from Addgene. Many others are also available under MTA by request at StrepStrains.
Plasmids used in Streptomyces
Plasmids used for PCR targeting (Redirect)
Redirect protocol and template information
Plasmids used for heterologous protein expression in E. coli
The following plasmids are frequently used to express proteins heterologously in E. coli, e.g. for two-hybrid analysis or protein purification.
Vectors for Bacterial two-hybrid experiments pKT25/pKNT25 | pUT18/pUT18C