Plasmids: Difference between revisions

From ActinoBase
No edit summary
No edit summary
Line 1: Line 1:
[[File:Actinobase-icons-trans_Plasmids.png|150px|Plasmids|link=Plasmids]]
[[File:Actinobase-icons-trans_Plasmids.png|150px|Plasmids|link=Plasmids]]


Various plasmids have been developed for use in <em>Streptomyces</em> (most will also contain an origin of replication that functions in <em>Escherichia coli</em>, for cloning, general maintenance, and [[intergenicconjugation into Streptomyces]]).
The following is an alphabetical list of a number of useful vectors - see also [[Table of Streptomyces vectors]].


==Plasmids used in <em>Streptomyces</em>==
==Plasmids used in <em>Streptomyces</em>==
Line 34: Line 37:




==Plasmids used in <em>Escherichia coli</em>==


==Plasmids used in <em>Escherichia coli</em>==
The following plasmids are frequently used to express proteins heterologously in <em>E. coli</em>.


[[pET28a]]
[[pET28a]]


[[pKNT25]] | [[pKT25]] | [[pUT18]] | [[pUT18C]]
[[pKNT25]] | [[pKT25]] | [[pUT18]] | [[pUT18C]]

Revision as of 15:35, 26 July 2019

Plasmids

Various plasmids have been developed for use in Streptomyces (most will also contain an origin of replication that functions in Escherichia coli, for cloning, general maintenance, and intergenicconjugation into Streptomyces).

The following is an alphabetical list of a number of useful vectors - see also Table of Streptomyces vectors.

Plasmids used in Streptomyces

pCAP03

pCRISPomyces2

pESAC13

pIJ733

pIJ8660

pIJ10257

pIJ12738

pKC1132

pMF96

pMS82

pRT802

pSET152

pSS170

Supercos1


Plasmids used in Escherichia coli

The following plasmids are frequently used to express proteins heterologously in E. coli.

pET28a

pKNT25 | pKT25 | pUT18 | pUT18C