Plasmids: Difference between revisions

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[[File:Actinobase-icons-trans_Plasmids.png|150px|Plasmids|link=Plasmids]]
[[File:Actinobase-icons-trans_Plasmids.png|150px|Plasmids|link=Plasmids]]


Various plasmids have been developed for use in <em>Streptomyces</em> (most will also contain an origin of replication that functions in <em>Escherichia coli</em>, for cloning, general maintenance, and [[intergenic conjugation into Streptomyces]]). Many of the plasmids commonly used <em>Streptomyces</em> are '''integrative''': using a [[site-specific phage recombinase]] they insert into a specific site on the <em>Streptomyces</em> chromosome.  
Various plasmids have been developed for use in <em>Streptomyces</em> (most will also contain an origin of replication that functions in <em>Escherichia coli</em>, for cloning, general maintenance, and [[intergenic conjugation into Streptomyces]]). Many of the plasmids commonly used <em>Streptomyces</em> are '''integrative''': they insert into a specific site on the <em>Streptomyces</em> chromosome using a [[site-specific phage recombinase]].  


The following is an alphabetical list of a number of useful vectors - see also [[Table of Streptomyces vectors]].  
The following is an alphabetical list of a number of useful vectors - see also [[Table of Streptomyces vectors]].  

Revision as of 15:59, 26 July 2019

Plasmids

Various plasmids have been developed for use in Streptomyces (most will also contain an origin of replication that functions in Escherichia coli, for cloning, general maintenance, and intergenic conjugation into Streptomyces). Many of the plasmids commonly used Streptomyces are integrative: they insert into a specific site on the Streptomyces chromosome using a site-specific phage recombinase.

The following is an alphabetical list of a number of useful vectors - see also Table of Streptomyces vectors.

Plasmids used in Streptomyces

pCAP03

pCRISPomyces2

pESAC13

pIJ101

pIJ8660

pIJ10257

pIJ12738

pKC1132

pMF96

pMS82

pRT802

pSET152

pSS170

Supercos1


Plasmids used for PCR targeting (Redirect)

pIJ773


Plasmids used for heterologous protein expression in E. coli

The following plasmids are frequently used to express proteins heterologously in E. coli, e.g. for two-hybrid analysis or protein purification.

pET28a

pKNT25 | pKT25 | pUT18 | pUT18C