Difference between revisions of "Plasmids"

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[[File:Actinobase-icons-trans_Plasmids.png|150px|Plasmids|link=Plasmids]]
 
[[File:Actinobase-icons-trans_Plasmids.png|150px|Plasmids|link=Plasmids]]
  
Various plasmids have been developed for use in <em>Streptomyces</em> (most will also contain an origin of replication that functions in <em>Escherichia coli</em>, for cloning, general maintenance, and [[intergenic conjugation into Streptomyces]]). Many of the plasmids commonly used for <em>Streptomyces</em> work are '''integrative''': they insert into a specific site on the <em>Streptomyces</em> chromosome using a [[site-specific phage recombinase]].  
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Various plasmids have been developed for use in <em>Streptomyces</em> (most will also contain an origin of replication that functions in <em>Escherichia coli</em>, for cloning, general maintenance, and [[intergeneric conjugation into Streptomyces]]). Many of the plasmids commonly used for <em>Streptomyces</em> work are '''integrative''': they insert into a specific site on the <em>Streptomyces</em> chromosome using a [[site-specific phage recombinase]].  
  
The following is an alphabetical list of a number of useful vectors - see also [[Overview of Streptomyces vectors]].  
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The following is an alphabetical list of a number of useful vectors - see also [[Overview of Streptomyces vectors]]. Some of these vectors are available under MTA from [https://www.addgene.org/ Addgene]. Many others are also available under MTA by request at [mailto:jic.strepstrains@jic.ac.uk|JIC StrepStrains].
  
 
==Plasmids used in <em>Streptomyces</em>==
 
==Plasmids used in <em>Streptomyces</em>==
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[[pCAP03]]
 
[[pCAP03]]
  
[[pCRISPomyces2]]
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[[pCRISPomyces-2]]
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[[pCRISPR-Cas9]]
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[[pCRISPR-dCas9]]
  
 
[[pESAC13]]
 
[[pESAC13]]
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[[pIJ12742]]
 
[[pIJ12742]]
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[[pIJ6902]]
  
 
[[pKC1132]]
 
[[pKC1132]]
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[[pSS170]]
 
[[pSS170]]
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[[pTOYAMAcos]]
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[[pTYM18]]
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[[pTYM19]]
  
 
[[Supercos1]]
 
[[Supercos1]]
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[[pGM190]]
 
[[pGM190]]
  
[https://link.springer.com/article/10.1007%2Fs00253-018-9334-5 pSG5]
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==Plasmids used for PCR targeting (Redirect)==
 
 
  
==Plasmids used for PCR targeting (Redirect)==
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[http://streptomyces.org.uk/redirect/index.html Redirect protocol and template information]
  
 
[[pIJ773]]
 
[[pIJ773]]
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[[pET28a]]
 
[[pET28a]]
  
[[pKNT25]] | [[pKT25]] | [[pUT18]] | [[pUT18C]]
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Vectors for Bacterial two-hybrid experiments
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[[pKT25/pKNT25]] | [[pUT18/pUT18C]]

Latest revision as of 17:41, 3 March 2022

Plasmids

Various plasmids have been developed for use in Streptomyces (most will also contain an origin of replication that functions in Escherichia coli, for cloning, general maintenance, and intergeneric conjugation into Streptomyces). Many of the plasmids commonly used for Streptomyces work are integrative: they insert into a specific site on the Streptomyces chromosome using a site-specific phage recombinase.

The following is an alphabetical list of a number of useful vectors - see also Overview of Streptomyces vectors. Some of these vectors are available under MTA from Addgene. Many others are also available under MTA by request at StrepStrains.

Plasmids used in Streptomyces

pCAP03

pCRISPomyces-2

pCRISPR-Cas9

pCRISPR-dCas9

pESAC13

pIJ101

pIJ8660

pIJ10257

pIJ12738

pIJ12742

pIJ6902

pKC1132

pMF96

pMS82

pRT802

pSET152

pSS170

pTOYAMAcos

pTYM18

pTYM19

Supercos1

pGM9

pGM190

Plasmids used for PCR targeting (Redirect)

Redirect protocol and template information

pIJ773


Plasmids used for heterologous protein expression in E. coli

The following plasmids are frequently used to express proteins heterologously in E. coli, e.g. for two-hybrid analysis or protein purification.

pET28a

Vectors for Bacterial two-hybrid experiments pKT25/pKNT25 | pUT18/pUT18C